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The findings were published recently in the Canadian Journal of Physiology and Pharmacology

Increased expression of neurokinin-1 has been detected in the intestines of patients with pseudomembranous colitis, highlighting its role in the pathogenesis of CDI [175]

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The samples were then washed with 1 PBS containing 1:500 RNAse inhibitor (Protector RNAse Inhibitor, Millipore Sigma) for 5 min at room temperature in triplicate