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MBP tags can help with solubility issues, but scientists may also choose to add smaller proteins, such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble

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Wei D A Versatile Trichoderma Reesei Expression System for the Production of Heterologous Proteins

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Methods 9 , 676682 (2012)

Given the above-described varied clinical presentations, especially in children, any unexplained severe neurological signs or symptoms should raise the suspicion of thiamine deficiency [see Hiffler et al
