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To obtain a homogenous cell population of human iPSCs for HLC differentiation, the cells were cultured in the Cellartis DEF-CS Culture System

doi: 10.1186/s12967-017-1175-y 96

Detergent-soluble extracts derived from SH-SY5Y neural cells transiently expressing YFP-tagged wild-type ArfGAP1 were subjected to co-immunoprecipitation (IP) assays using anti-GFP antibody to enrich for interacting partners of ArfGAP1 and submitted for LC-MS/MS analysis