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6d, e) under two distinct assumptions: the common effector assumption and the distinct mechanisms assumption (see schematic representations in (Fig

DIA files were processed using Spectronaut with default settings, with PTM localization activated and site confidence score cutoff set to 0 (for peptide-level analysis of dilution benchmark), 0.75 (for spectral library analysis) or 0.99 (for direct DIA analysis), data filtering set to Q- value and Normalization Strategy set to Local Normalization

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The exact mechanisms, sites, and pathways of action of CNS-targeted IN therapeutics are still under debate, but IN drugs and tracer molecules appear to be distributed throughout the brain via the CSF and extracellular fluid of the perivascular spaces around the olfactory and trigeminal nerves [14]

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