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All these results allowed to conclude that APR-246 induces early cell death by ferroptosis, a recently described non-apoptotic form of regulated cell death that links together membrane lipid peroxidation, cysteine and iron metabolism, glutathione peroxidase activity and oxidative stress (as summarized in Figure 4A 2325 )

Staining in shoot was much lower and only restricted to leaf tip for NBT ( Supplemental Figure 2C )

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Vilon's effects on chromatin remodeling and gene expression begin at the cellular level during administration, but noticeable systemic changes typically require multiple cycles over several months