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Description
From a mechanistic perspective, five classes of inhibitors of SIRT1 have been identified: First, competitive inhibitors that vie for acylated substrates at the binding sites, exemplified by natural products such as sirtinol, splitomicin, and cambinol analogs

Marra, F., Gastaldelli, A., Svegliati Baroni, G., Tell, G

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Moreover, ROS serves as signaling molecules that enhance cellular proliferation and survival pathways

References BJMO

For transfection (all volumes per dish), 4 L of Lipofectamine RNAimax (Life Technologies) was combined with 250 L OPTI-MEM (Life Technologies) and separately 30 pmol of siRNA was combined with 250 L of OPTI-MEM
