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Compared to control cells, IRE1-null MDA-MB-231 cells displayed attenuated lipid peroxidation upon erastin treatment, whereas reconstitution with wild-type IRE1 but not the K907A mutant restored lipid peroxidation (Fig

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Herein, the mh-Tyr crystal structure was also processed by deletion of co-crystallized ligand and water molecules, the addition of polar hydrogen atoms, optimization of hydrogen-bonding network rotation of thiol and hydroxyl hydrogen atoms, tautomerization and protonation states for histidine (His) residue, assignments of Chi flip for asparagine (Asn), glutamine (Gln), and His residues, and optimization of hydrogen atoms in distinct species achieved by the Protein preparation wizard

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