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Wounds displayed faster healing, decreased concentration of metalloproteinases 2 and 9 as well as of TNF- (a major inflammatory cytokine) compared with vehicle alone or with untreated wounds [25]

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At the end of the experiment, RNA was extracted from the cells, and RT-qPCR analysis of gene expression was carried out using specific primers against rat fibroblast-specific protein-1 (FSP1), keratin-19 (KRT19), aggrecan, and type I and type II collagen

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