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slope = 1.0116, R 2 = 0.9924 for T 2 )

However, these studies found that copper inhibited GABA A Rs at nanomolar (IC 50 = 35 nM) rather than micromolar concentrations (Sharonova et al., 1998)

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Once the fusion protein is expressed and purified, tags like GFP and 6 His, incorporated into the AMP sequence, further facilitate detection and purification ( Figure 6b )