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The final purified products were in 20 mM Tris-Cl buffer, pH 8.5 at a final concentration of 1.0 mg/ml quantified by amino acid analysis

While the [U- 13 C]serine tracing showed SAAR increased labeling of other fates of cysteine such as taurine, loss of hepatic PSAT1 uniquely attenuated the increased liver 13 C-GSH and 13 C-GSSG from [U- 13 C]glucose

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202526, BioLegend) and Zombie near-infrared (NIR) fixable viability dye (1:1,000

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Taken together, our study refines the critical Gln/c-Myc/miR-29b-3p/GGCT regulatory axis, clearly illustrating how Gln modulates GGCT expression