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The reaction mixture (1.0 mL) consist of 0.4 mL acetate buffer (0.2 M, pH 4.8), 0.2 mL of sucrose (0.4 M) and enzyme extract of about 0.4 mL, was incubated for 1 h at 30 C

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Naito C, Kajita M, Niwa T (1999) Determination of glutathionyl hemoglobin in hemodialysis patients using electrospray ionization liquid chromatography-mass spectrometry
