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Clin Dermatol 2022;40:103-13

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

S.BharathB

This divergence in clinical course parallels has been observed in human influenza, in which young persons without significant comorbidities tend to recover by Day 7, whereas older persons with comorbidities often clinically deteriorate