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Ltd., Shanghai, China) for cell nuclei staining

Separation was achieved using a linear gradient from an initial oven temperature of 50 C at 3 C/min to a final temperature of 350 C, which was maintained for 5 min

1 Introduction With an aging global population, it is estimated that 2.2 billion people worldwide are suffering from visual dysfunction, which severely impedes the individuals quality of life and poses threats to public health ( Ferroptosis, a term initially coined by Dixon in 2012, is a distinctive form of RCD featured by the iron-dependent lethal aggregation of lipid peroxides located at the cell membrane ( Figure 1 )

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