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Cells were cultured for 72 h with medium replenished every 24 h

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doi: 10.1300/J092v08n03_10 352 KennedyGNorrisGSpenceVMcLarenMBelchJJ

We investigated the presence of Kupffer cells within the organoids using the marker CD68 61 and observed a CD68 + population exhibiting typical cytoplasmic granule staining (Fig