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Separation of the target protein and the GST moiety can be achieved by re-chromatography on the glutathione Sepharose column (after dialysis to PBS buffer) to remove the GST and any un-cleaved fusion protein

cerevisiae strain BY4741 was transformed with full-length of P450 genes AG1IA_05136 , AG1IA_07929 , AG1IA_01023 , AG1IA_06336 , and AG1IA_07129 and GST gene AG1IA_07383

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