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Nucleosides were separated by reversed-phase ultra-performance liquid chromatography on a C18 column, coupled to mass spectrometry detection with an Orbitrap Fusion Tribrid LC mass spectrometer (Thermo) in positive electrospray ionization mode

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ROS may include singlet or triplet oxygen, but the major contributor is the superoxide anion O 2

International Journal of Molecular Sciences Obeid R, et al