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At the end of the incubation of 30 min or 90 min, some of the MCTS were transferred to a new agarose-coated 96-well plate, and cultured in drug-free medium for different lengths of time (2, 6, 12, and 24 h) to further study the post treatment effects and drug elimination

G.SinningI.Alwyn JonesT.et al (1995a)

SeleniumA Trace Element of Clinical Significance

Sci Total Environ 807:150455

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The study recommends further investigations with different dosing frequencies of VCN, a combination of VCN, and PFD in different doses to evaluate the possible synergism or additive effects