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Stress-induced Ca 2+ spikes serve as secondary messengers, activating calcium-dependent protein kinases (CDPKs) and calcineurin B-like proteins (CBLs) that regulate stress-responsive gene expression ( 2+ -mediated pathways by stabilizing ATP-dependent pumps and channels, ensuring controlled Ca 2+ fluxes during stress responses

[DOI] [Google Scholar] Bergstrm S

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34 The same glutathione Sepharose column may be used for both initial isolation of the fusion protein and for repurification after enzymatic cleavage

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