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For most people, B12 injections are safe and well-tolerated

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Diabetologia 63 , 15421553 (2020)

(Animals were secondarily euthanized via cervical dislocation.) Whole blood was transferred into two separate micro-collection tubes (heparin-coated micro-collection tube: BD Microtainer item 13-680-62

Purified GST-MFAP2 (full-length) was immobilized on GlutathioneSepharose 4B beads by incubating 20 g of the protein with 30 L of pre-washed beads in binding buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM DTT, and 0.1% Triton X-100) (Beyotime, Shanghai, China) for 1 h at 4 C with gentle rotation

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