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Dehydration was carried out using ascending alcohol grade i.e ., 50, 70, 80, 90 and 100% alcohol after which clearing was done with propylene oxide

This allows assessment of tolerance before increasing to standard therapeutic doses of 1-2mg daily

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doi: 10.18632/oncotarget.18175 30 WildeLRocheMDomingo-VidalMTansonKPhilpNCurryJet al