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When serum B12 falls in the gray zone, methylmalonic acid testing is recommended to clarify whether a functional deficiency exists

The primary preclinical evidence comes from mouse studies showing reduced body weight and adipose mass in diet-induced obese mice (2018), improved grip strength in aged mice (2024), and beneficial microbiome changes when combined with caloric restriction (2022)

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Preparation and serial dilution of compound stock solutions

Prick testing was negative (Table 1a), but intradermal testing to all cyanocobalamin prepara- tions and benzyl alcohol was positive (Table 1b)

CSF Oxytocin in Anorexia Nervosa and Bulimia Nervosa: Clinical and Pathophysiologic Considerations