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Description

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Using human liver microsomes, it was also recently reported that sulforaphane metabolites (0-200 M) had little-to-no effect on the activities of CYP1A2, CYP2B6, CYP2D6, and CYP3A4 (23)

The quantification of GSH was carried out by comparing the fluorescence produced by the samples versus that of a standard solutions curve with known concentrations of GSH.11 To measure GSSG concentration, 25L aliquots of saliva were incubated with 10L of a N-ethylmaleimide solution (NEM, 5mg/mL in distilled water) for 40min at room temperature