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For experiments involving intracellular staining of cytokines, T cells were stimulated for 3 h (mouse) and 6 h (human) with phorbol 12-myristate 13-acetate (PMA

doi: 10.1007/s12035-020-02212-w

Unlike many peptides that are synthesized and secreted by endocrine glands, MOTS-c originates directly from the mitochondria, highlighting its intimate connection to cellular energy processes

In addition, the metabolites had no significant differences on d 65

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