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Analytical verification requires reverse-phase HPLC with UV detection at 214-220 nm to confirm retention time consistency and peak purity above 95%, while mass spectrometry (MALDI-TOF or ESI-MS) can confirm the expected molecular ion at 1419.53 Da and identify any truncation or oxidation products

Importantly, because Dihexa promotes true structural changes in synaptic connections rather than temporary stimulation, improvements often persist beyond treatment cessation

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