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For validation and sample analysis using the multiple standard addition approach, a series of spiking mixture solutions (low, medium, and high concentrations for validation, and adjusted concentrations for sample analysis) were prepared

Li Y, Leung PSC, Gershwin ME, Song J (2022) New Mechanistic Advances in FcRI-Mast Cell-Mediated Allergic Signaling

It's a question of goals

The question of whether you can take these two together is usually met with a "yes" because of how they balance each other out
Single-cell analysis identifies distinct macrophage phenotypes associated with prodisease and proresolving functions in the endometriotic niche

Rheumatology International , 33(1), 167-172