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After initial dialysis, multi histidine-tagged receptor proteins were purified with immobilized metal affinity column chromatography and the peak fractions were followed by size exclusion column chromatography as previously described [23]
doi: 10.1038/s41430-024-01451-7
As a result, heme concentration reached 29.57 2.46 mg/L (Ko et al
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Almeida, A., Moncada, S
