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In this review, we focus on three specific methods that have been used to study the neuroimmunology of ME/CFS: positron emission tomography (PET) using translocator protein (TSPO) binding radioligand, magnetic resonance spectroscopy (MRS), and assays measuring cytokines in blood and cerebrospinal fluid We offer a particular focus on what can and cannot be concluded by studies using these methods

10.1038/emboj.2013.74 EMBO J

Carbon dioxide/bicarbonate is required for sensitive inactivation of mammalian glyceraldehyde-3-phosphate dehydrogenase by hydrogen peroxide

In another work, Gondim et al

In other words, measuring the same sample twice with the same kit in the same laboratory following the same strict protocol yielded significant differences in absolute cytokine values (Figure 2)

Their movements including smiling and blinking