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Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs

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PGPIPN regulated contents and/or activities of ACC, PPAR-, CHOP and Caspase-3 proteins related with lipid metabolism and oxidative stress in hepatic cells Western blotting was used to analyze ACC, pACC (phosphorylated ACC), PPAR-, CHOP, Caspase-3 and Cleaved caspase-3 proteins in both model cells and model animals (Figure 8)

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