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Chen Q, Ke H, Dai Z, Liu Z (2015) Nanoscale theranostics for physical stimulus-responsive cancer therapies

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To evaluate the long-term effects of different compounds on cell proliferation, 2D clone formation assay was performed as previously described

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If you're very low in B12, you can get higher-dose vitamin B12 shots

By comparing this with the total amount added in the first place, you can then calculate how much of the ligand that has been bound to the beads
