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doi: 10.3389/fimmu.2025.1477437 Received 07 August 2024 Accepted 20 January 2025 Published 10 February 2025 Volume 16 - 2025 Edited by Nejat Dalay, Istanbul University, Trkiye Reviewed by Junwei Luo, Henan Polytechnic University, China Mario Perez-Medina, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico Updates Copyright 2025 Hu, Tian, Liu, Hou, wu, Wang and Shu

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The column was then washed with 5 mls of equilibration buffer before being washed with 3 5 mls of wash buffer (50 mM Tris, 300 mM sodium chloride, 20 mM immidazole, pH 8.0), before elution with 2 400 l of elution buffer (50 mM Tris, 300 mM sodium chloride, 250 mM immidazole, pH 8.0)

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