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Cells were cultured in DMEM lacking glucose (Thermo Fisher Scientific, 11-966-025) with 10% dialyzed FBS (Thermo Fisher Scientific, 26400044), 100 g/mL of uridine (Sigma-Aldrich, U3750), 1 mM pyruvate (Thermo Fisher Scientific, 11360070), and either 10 mM glucose (A2494001) or 10 mM galactose (Sigma-Aldrich, G5388-100G)

The additional substances tested include: Barbiturates like phenobarbital and secobarbital Benzodiazepines including Valium, Xanax, and Klonopin Methadone Methaqualone (though often replaced with oxycodone screening) Propoxyphene Again, no peptides

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