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Normal dermal fibroblasts were seeded into a 96-well cell culture plate and have been grown to confluence in high glucose media, supplemented with 10% fetal calf serum for around 3 days

10 M retinoic acid was used as a positive control for maturation towards a differentiated neuronal phenotype, as shown previously ( 2.2 DPI synergizes with MitoQ in MNA NB cells To assess whether MitoQ and DPI act synergistically in reducing cell viability, we treated two MNA NB cell lines (IMR-5/75 and Be(2)-C) with various concentrations of DPI and MitoQ

Researchers hypothesize that the elevated NAD+ levels increase futile cycling a process where cells burn energy to maintain electrochemical gradients rather than storing it as lipid droplets

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