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After 18 h, the cells in upper chamber were fixed in methanol and underwent crystal violet staining

These interconnected mechanisms further illuminate the complexity of ferroptosis regulation and underscore the essential role of lipid peroxidation in its execution

have demonstrated equivalent activity after lyophilization and reconstitution of O 2 -tolerant [NiFe]-hydrogenases to freshly isolated enzyme, indicating that metal cofactors and amino acid side-chains responsible for proton/electron transfer were not altered by lyophilization 36