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Peroxidase and non-specific protein blocking were done as per the instructions using the Abcam ABC detection kit (ab64264) and incubated with the following primary antibody dilutions: CHG (Cell Signaling, 70076), SYP (Abcam, ab178945) and MYCN (Cell Signaling, 4370) with 1:150 dilution

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The biomarkers in blood and urine are outlined below

The Connectivity Map database documents broad regulatory activity involving repair, antioxidant, and anti-inflammatory pathways

Depending on individual skin responses, maintenance sessions may be needed every 6-12 months

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