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doi: 10.1093/nar/24.21.4242

5:24 Liu, X

For comparison, we also simulated conventional centromeric (PDB 6O1D) (61) and noncentromeric nucleosomes (PDB 3LZ0) (62) on 601 superpositioning sequence (63), using 3D models from, respectively, cryo-EM data (3.4 resolution) and x-ray diffraction (2.5 resolution) as starting coordinates

contributed to the conception of the study and wrote the initial draft

As these were already lower in the mutants compared to the WT this led to stronger phenotypes regarding root length and total biomass production

Once you have gathered the material containing the protein you want to study it is necessary to generate a crude extract -- for proteins from muscle that would mean grinding it up, for an intercellular protein that would mean breaking the cells open, etc