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The rectal temperature of each piglet was measured every 12 h after inoculation, while the rectal swabs of each piglet were collected every 12 h for detection of viral shedding via real-time RTqPCR (the TaqMan probe used for detecting PEDV is presented in Table 2)

THP-1 cells were differentiated into macrophages by 100 nM of phorbol-12-myristate-13-acetate (Sigma-Aldrich), and differentiated into M1 by adding LPS (100 ng/mL, Sigma-Aldrich) and IFN- (20 ng/mL, R&D Systems) for 24 h, and M2 cells with M-CSF (20 ng/mL, Peprotech), IL-4 (40 ng/mL, R&D Systems), and IL-13 (40 ng/mL, R&D Systems) for 4 days

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PMID: 93 ParkJRLeeMCMoonSCKimJHaKTParkEJet al