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By the early 21 st century, cumulative experimental and clinical evidence converged into a coherent, evidence-based pathogenesis, substantiating the hypothesis about the pivotal role of the colonic epithelium in the development of this disease

A gradient LC elution program was utilized for mobile phases consisting of 0.1% formic acid (0.1% v/v) in ultrapure water (buffer A), and acetonitrile plus 0.1% (v/v) formic acid (buffer B) at a flow rate of 0.4 mL/min

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The pathogenesis encompasses a wide range of biological processes, including systemic inflammation, altered metabolism, and immune dysregulation

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