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They found that infusion of D-BHB extended lifespan by nearly 30% and ameliorated the severity of motor deficits, supporting the idea that improving the source of cellular energy may enhance neuronal survival and improve functional outcomes in HD models [96]

Protein samples were mixed with SDS sample buffer and boiled for 5 min

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Trypsin (10 ng/ul) dissolved in 25 mM ammonium bicarbonate was added to the gel pieces (typically 200 ng of protease) and incubated at 37 C for 16 h

This has been attributed to oxPTMs and the subsequent neoepitopes influencing several immunological phenomena, including epitope spreading, molecular mimicry, antigen coupling, and exposing of cryptic epitopes, with these mechanisms influencing the loss in immune tolerance