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The reaction was followed and upon completion analyzed by 1 H NMR

Winderickx J, de Winde JH, Crauwels M, Hino A, Hohmann S, van Dijck P, Thevelein JM (1996) Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae : novel variations of STRE-mediated transcription control

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[24] This complex is too large to cross the capillary endothelium freely, so it functions as a slowly releasing circulating reservoir: it prolongs the systemic presence of IGF-1 while simultaneously keeping the bulk of it biologically restrained until it is handed off to tissues

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