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Having incubated the immunoprecipitated samples overnight at 4 C with rotation, the anti-M2 FLAG antibody-conjugated agarose beads were washed three times (each for 5 min) with ice-cold washing buffer (50 mM Tris, pH 7.4, 137 mM NaCl, 10% glycerol, 1% NP-40)

Chang RYK, Chan H-K

Lee WM (2017) Acetaminophen (APAP) hepatotoxicityisnt it time for APAP to go away

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The hallmark symptom is stabbing pain near the heel, particularly intense with the first steps after waking or after prolonged periods of sitting

doi: 10.1161/01.res.0000012569.55432.02