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The stemness-related gene ( Sox2 , Nanog and Oct4 ) were quantified by qPCR analysis
Effect of a butyrate-fortified milk replacer on gastrointestinal microbiota and products of fermentation in artificially reared dairy calves at weaning

Agilent Technologies, Wilmington, DE) coupled to a tandem mass spectrometer (HP 1100 series MSD

When intracellular glutathione levels are sufficient, this feedback mechanism suppresses further synthesis, preventing excessive accumulation of pathway intermediates

Additionally, genes implicated in the AMPK signaling pathway, including cystic fibrosis transmembrane conductance regulator ( Cftr ) and protein kinase AMP-activated non-catalytic subunit gamma 3 ( Prkag3 ), along with those associated with oxidative phosphorylation, such as cytochrome c oxidase subunit 6a2 ( Cox6a2 ) and ATPase H + transporting v1 subunit g3 ( Atp6v1g3 ), were significantly downregulated

Statistical Analysis Each experiment was done in duplicate and repeated a minimum of three times
