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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]

A: Retatrutide activates three receptors (GLP1, GIP, and glucagon)

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[DOI] [PubMed] [Google Scholar] 67.Dong S., Sun C
10.1038/nrd.2017.116 [DOI] [PMC free article] [PubMed] [Google Scholar] 19.van Deursen JM
