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Once the experiment had ended, after recording the ethanol intakes at the second day (day 63) of alcohol relapse, the animals were anesthetized with chloral hydrate (280 mg/kg, i.p.) and euthanized to obtain hippocampal samples for determination of GSSG and GSH and astrocyte and microglia immunoreactivity

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MTHFR, or the methylenetetrahydrofolate reductase is the key regulatory enzyme in folate and homocysteine metabolism

Follow-up sessions are typically scheduled every 4 to 6 weeks during the initial series

4.2.4.3 Temperature-responsive nanoparticles In DDS, nanoparticles can be engineered to exhibit temperature-responsive characteristics, including temperature-induced phase transitions, conformational changes, drug release, and structural transformations (Dai et al., 2025)

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