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The phage pellet was resuspended in phage buffer (68 mM NaCl, 10 mM Tris HCl pH 7.5, 10 mM MgSO 4 , 10 mM CaCl 2 ) and cesium chloride (CsCl) was added to a density of 1.5 g cm 3 (4.1 M), and subjected to equilibrium density gradient centrifugation at 132,600 x g for 16 h
The remaining layers are conditional added only when a specific bottleneck shows up, not by default: Selank fits when stress-triggered flushing rides alongside reactive skin
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