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Expert opinion on therapeutic targets

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The gels were equilibrated in transfer buffer (48 mM Tris, 39 mM glycine, 20% methanol) and proteins were transferred to nitrocellulose membranes on Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (BioRad) 15 V for 1 h

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Screening for occult malignancies may be appropriate before starting long-term protocols
