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The lysate with LLO was loaded onto 30 ml Ni-NTA Superflow resin (QUIAGEN, Germany) packed in a XK 16/200 column (GE Healthcare, USA), washed with wash buffer (20 mM MES/NaOH pH 6.5, 150 mM NaCl), and eluted with an imidazole gradient in 20 mM MES/NaOH pH 6.5, 150 mM NaCl, with 500 mM imidazole as the final concentration

PMID: 26216348 Cui YX, Du JB ZQY, Liao Y, Liu P, Wang YL, Qi JG, Yan H, Xu WR, Liu XQ, Sun Y, Sun CF, Zhang CY, Chen YH, Jin HF