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Closer examination revealed 211 DEGs when comparing the GR mutant strain to the WT strain, with 123 genes upregulated and 88 genes downregulated in the GR mutant strain (Figure 3B)

Other amino acids, such as glycine and alanine, along with anabolic hormones, such as insulin, promoted hepatocyte swelling, leading to increased biosynthetic processes [77], while catabolic hormones, like glucagon, reduced intracellular glutamine levels induced hepatocyte shrinkage [78]

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Continued daily use maintains the collagen density achieved, prevents further degradation, and continues the gene-level anti-aging effects that slow the rate of future aging