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Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 m Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare)

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Endoperoxides was separated on a Luna C18 reverse phase column, 250 4.6 mm, 5 M particle size (Phenomenex, Torance, CA) and monitored at 210 nm

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This also elaborates that one can not sell peptides as a sports or health supplement