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Concurrently, lack of Nrf2 decreased protein aggregation and cardiac hypertrophy and improved cardiac contractility in the mice transgenic for the mutant human B-crystallin, suggesting that the sustained upregulation of Nrf2 and subsequent increase in GSH-mediated removal of hydrogen peroxide contributes to the reductive stress phenotype [51]

The advantage of FLIM is that it is usually not affected by the concentration of the fluorophore and by photobleaching ( ExDonor ) energy transfer occurs to the acceptor molecule with concomitant decrease in the donors emitted fluorescence ( EmDonor ) and increase in the acceptors emitted fluorescence ( EmAcceptor) )

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[DOI] [PMC free article] [PubMed] [Google Scholar] 188.Crandall J., Sakai Y., Zhang J., Koul O., Mineur Y., Crusio W.E., McCaffery P