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BER solution, F3 and F5, each containing a quantity of berberine corresponding to 2.5 mg, were then put in stoppered glass vials containing 10 ml of acetate buffer (pH 5) as a dissolving medium [21]

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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

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Biofactors 42: 431440, 2016

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